bsk6 slice keeper  (Scientific Systems Design Inc)

Journal: The EMBO Journal

Article Title: The TrkC-PTPσ complex governs synapse maturation and anxiogenic avoidance via synaptic protein phosphorylation

doi: 10.1038/s44318-024-00252-9

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Acute 400 μm slices containing dorsal hippocampus were obtained using a VT1200S vibratome (Leica Biosystems) and then transferred to an incubation chamber (BSK12, Scientific Systems Design) containing a standard artificial cerebral spinal fluid (ACSF) comprised of (in mM): 124 NaCl, 10 d -glucose, 26 NaHCO 3 , 2.5 KCl, 1.25 NaH 2 PO 4 , 2 CaCl 2 and 1 MgSO 4 at pH of 7.3–7.4 and osmolarity of 290–300 mOsm/L.

Techniques: Knock-In, Recombinant, Plasmid Preparation, Clone Assay, Staining, Sequencing, DC Protein Assay, Membrane, Western Blot, Electron Microscopy, Mass Spectrometry, Phospho-proteomics, Software, Fluorescence, Microscopy, Incubation